KIT antibody

Catalog No. ABIN371419

This Mouse Monoclonal antibody specifically detects KIT in FACS. It exhibits reactivity toward Chicken.

Quick Overview for KIT antibody (ABIN371419)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIT antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: Kit2c75

Product Details anti-KIT Antibody

Specificity: This antibody recognizes Chicken c-Kit.

Characteristics: Synonyms: SCFR, KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase

Purification: Purified

Immunogen: Sp2/0 cells, Sp/chkit4A4, transfected with Chicken c-kit cDNA. Remarks: The amino acid sequence of Chicken c-kit shows 63% identity to Mouse c-Kit andexhibits similar expression patterns in RNA blot analysis suggesting similar biologicalroles. (Ref. 1)

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.6-10)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: CD117 / C-Kit

Background: C-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR

Gene ID: 378783

UniProt: Q08156

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity